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Detection of meningococcal serogroups A, Y, W135 and pneumococcal bacteria by ultrasound latex agglutination; Comparison with PCR

  • Researchers:
    Dr Ed Kaczmarski, Dr Rosemary Barnes, Dr W Coakly
  • Start Date:
    01 January 2000
  • Category:
  • Location:
    Cardiff University, Cardiff, UK

The serogrouping of meningococcal groups A, Y and W135 in clinical blood samples will be investigated by ultrasonic standing wave agglutination of commercially available and in-house prepared individual antibody-coated latex bead suspensions. Conditions for detection of pneumococci, and Strep agalactiae (Lancefield group B) by ultrasonic agglutination of commercially available latex kits kits will be optimised. In further experiments, latex particles will be coated by monocional antibody to the pneumococcal toxin, pneumolysin. The lower limits of pneumococcal bacteria detectable by ultrasonic agglutination of these particles will be established and compared with detection of currently available beads which are coated with polyclonal antibody to capsular polysaccharides. The sensitivity and specificity of the ultrasonic technique in clinical samples containing the above antigens will be compared with PCR detection of bacterial DNA in samples obtained by Manchester PHLS Meningococcal Reference Unit (MRU).

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